Fig. 4.

Analysis of sucrose-density gradient trace of active T. maritima AP (4), represented by a thick line. The standards carbonic anhydrase (1), E. coli AP (2) and aspartate transcarbamoylase (3) are shown in thin lines. Sucrose-density gradient sedimentation experiments were performed according to the protocol in Materials and Methods. Absorbance measurements were taken continuously as the protein-containing solution was pumped through a UV detector.