Fig. 4.

Mono Q ion-exchange chromatography separates cytoplasmic dynein intermediate chain (IC) into two biochemically distinct pools. (A) Elution profile of Mono Q column. Left axis = A₂₈₀, right axis = KCl gradient used for elution. (B) Coomassie brilliant blue stained 15% acrylamide gel of fractions (indicated by numbers). L = column load. The lines on the right indicate positions of presumptive light chains (LCs) described in the text. Molecular weight markers are indicated to the left. (C) Silver-stained, two-dimensional gel analysis of IC polypeptides from the IC– and IC/LC pools. The regions containing the IC polypeptides are shown with the acidic end of the IEF gels to the left. Arrows indicate the positions of the IC2C isoform found in both IC pools and the IC1 isoform found predominantly in the IC/LC pool.