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Fluorescence spectra of dansyl-SC3 in different conformations. Thick solid line: soluble-state dansyl-SC3 in buffer. Thin solid line: the α-helical-state dansyl-SC3 on Teflon. Dotted line: the β-sheet-state dansyl-SC3 on Teflon after 1-h incubation at 65°C in the presence of 0.1% (v/v) Tween 80. Dashed line: free dansyl reagent in buffer. Dashed-dotted line: dansyl-labeled angiotensin I. The spectra were corrected for the background and the volume changes.
