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. 2002 May;11(5):1172–1181. doi: 10.1110/ps.4530102

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2002, © Oxford University Press

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Fig. 6. — FRET on the β-sheet-state dansyl-SC3. (A) Solid line: fluorescence spectrum of TFA/dansyl-SC3 + TFA/dabcyl-SC3 (molar ratio 1:1) in buffer. Dotted line: the same sample supplemented with Teflon spheres and heated up to 65°C. Dashed line: the same sample supplemented with 0.1% (v/v) Tween 80 and incubated to 15 h. The spectra were corrected for the background signal and the changes of volumes. (B) The kinetic fluorescence recorded at 520nm. Thick solid line: fluorescence change of β-sheet-state TFA/dansyl-SC3 + TFA/dabcyl-SC3 (molar ratio 1:1) on Teflon. Thin solid line: β-sheet-state TFA/dansyl-SC3/dabcyl-SC3 (molar ratio 1:1) on Teflon. Dotted line: β-sheet-state dansyl-SC3 on Teflon. The decrease in fluorescence could be fit to two exponentials, with a lifetime t₁ value of 2.5 min and a lifetime t₂ of 278 min. Beta-sheet-state SC3 was induced by heating to 65°C in the presence of 0.1% (v/v) Tween 80. Dashed line: α-helical-state dansyl-SC3 on Teflon induced by adding Teflon spheres. F0 indicates the initial fluorescence recorded at time 0.