Table 1.

Stern-Volmer constants for collisional quenching of dansyl-labeled SC3 in different conformations

	Ksv (I+)(M−1)		Ksv (Cs+)(M−1)	Ksv (acrylamide)(M−1)
Conformation	10 μg/mL	2 μg/mL	10 μg/mL	10 μg/mL	2 μm/mLa
Soluble-state	1.73	4.64	2.14	4.64	9.91
α-helical-state	no resultb		1.38	0.02
β-sheet-state, 15-min incubationc	no result		0.13	0.27
β-sheet-state, 30-min incubationc	no result		0.10	0.60
β-sheet-state, 6-h incubationc	no result		0.15	0.23

^a Different concentrations of dansyl-SC3.

^b Teflon spheres tended to congeal under the experimental condition, and therefore no reliable results were obtained.

^c β-sheet-state formation was stopped at the indicated times by cooling down the samples from 65°C to 25°C (de Vocht et al., unpublished).