Table 2.

Comparison of the thermodynamic parameters of the interaction of E3 and E1 with the PSBD of E2

Enzyme	Kaa (M−1)	ΔG°b (kcal/mol)	ΔH°c (kcal/mol)	TΔS°d (kcal/mol)	ΔCpc (kcal/molK)	Protons linkedc (n)
E3	1.7 × 109	−12.6	+2.2	+14.8	−316	−0.02
E1	3.1 × 109	−12.9	−8.4	+4.5	−470	+0.11

All the kinetic and thermodynamic parameters are taken from experiments in HBS buffer, pH 7.4 at 25°C.

^a Obtained from SPR analysis.

^b Calculated from ΔG° = −RT lnK_a.

^c Calculated from ITC measurments.

^d Calculated from TΔS° = ΔH° − ΔG°.