Fig. 2.

CD spectra of SC3 adsorbed on Teflon before and after addition of detergent. The changes in secondary structure of adsorbed SC3 upon treatment with Tween-20 were followed with CD spectroscopy. The spectra for monomeric SC3 (dotted line) and after adsorption to colloidal Teflon (dashed line) were reported previously (de Vocht et al. 1998). Addition of Tween-20 to the adsorbed SC3 at 25°C results in detachment and monomerization of the protein (thin line). Addition of Tween-20 at 85°C results in a conformational change to the β-sheet state (thick line).