Proposed secondary structures and domains of the b subunit and truncations. (A) The predicted secondary structure for the b subunit is indicated as α-helix, bend, or turn and the solution structure of the b2–34 construct (Dmitriev et al. 1999). (B) The domain structure proposed from deletion analysis (Revington et al. 1999) includes the transmembrane, tether, dimerization, and δ-binding domains as shown. (C) The constructs of the b subunit used in this study are illustrated, showing their sequence relationship to the domains outlined in panel B.