Fig. 5.

Concentration dependence of the ellipticity for the b_53–122 protein. The plot shows the mean residue ellipticity at 222 nm (θ₂₂₂) vs the protein concentration. The concentrations ranged from 0.5–50 μM in 25 mM Na₂HPO₄, pH 7.5 and 5°C. The curve shown is the best fit line according to equation 2 for a folded dimer (F₂) to folded monomer (F) equilibrium yielding a K_d = 0.6 ± 0.2 μM.