Table 1.
Spectral properties and SI values of NBD and dansyl-labeled PAI-1 species
| Raw data | Data corrected for SI | ||||||
| Position and fluorophore | λmax | Fluorescence intensity at λmax | SI | λmax | Fluorescence intensity at λmax | % Change | |
| 119 dansyl | Native | 495 ± 1 | 0.55 ± 0.03 | ||||
| Cleaved | 493 ± 2 | 0.56 ± 0.06 | |||||
| Trypsin | 492 ± 0 | 0.58 ± 0.02 | 2.0 | 488 ± 2 | 0.61 ± 0.07 | 9 | |
| LMW u-PA | 493 ± 1 | 0.56 ± 0.03 | 1.2 | 491 ± 3 | 0.58 ± 0.05 | 3 | |
| HMW u-PA | 494 ± 2 | 0.57 ± 0.03 | 1.2 | 491 ± 5 | 0.63 ± 0.05 | 13 | |
| t-PA | 489 ± 1 | 0.64 ± 0.04 | 1.3 | 484 ± 1 | 0.71 ± 0.06 | 27 | |
| 302 dansyl | Native | 497 ± 2 | 0.51 ± 0.03 | ||||
| Cleaved | 496 ± 3 | 0.42 ± 0.04 | |||||
| Trypsin | 487 ± 1 | 0.69 ± 0.04 | 2.2 | 486 ± 1 | 0.97 ± 0.09 | 131 | |
| LMW u-PA | 488 ± 3 | 0.71 ± 0.05 | 2.0 | 482 ± 1 | 1.07 ± 0.1 | 155 | |
| HMW u-PA | 485 ± 1 | 0.70 ± 0.07 | 1.9 | 481 ± 1 | 1.02 ± 0.2 | 143 | |
| t-PA | 480 ± 1 | 0.82 ± 0.03 | 1.8 | 475 ± 2 | 1.22 ± 0.09 | 190 | |
| 119 NBD | Native | 546 ± 2 | 0.51 ± 0.02 | ||||
| Cleaved | 535 ± 1 | 1.03 ± 0.08 | |||||
| Trypsin | 536 ± 1 | 0.89 ± 0.03 | 2.4 | 534 ± 2 | 0.87 ± 0.2 | −15 | |
| LMW u-PA | 538 ± 0 | 0.75 ± 0.04 | 1.1 | 538 ± 1 | 0.79 ± 0.06 | −23 | |
| HMW u-PA | 537 ± 2 | 0.76 ± 0.06 | 1.1 | 534 ± 2 | 0.95 ± 0.1 | −8 | |
| t-PA | 538 ± 0 | 0.75 ± 0.04 | 1.1 | 535 ± 1 | 1.03 ± 0.1 | 0 | |
| 302 NBD | Native | 548 ± 1 | 0.51 ± 0.02 | ||||
| Cleaved | 554 ± 3 | 0.40 ± 0.04 | |||||
| Trypsin | 548 ± 2 | 0.34 ± 0.04 | 2.0 | 545 ± 2 | 0.24 ± 0.08 | −40 | |
| LMW u-PA | 542 ± 1 | 1.14 ± 0.07 | 1.3 | 542 ± 1 | 1.62 ± 0.1 | 305 | |
| HMW u-PA | 544 ± 2 | 1.1 ± 0.1 | 1.3 | 543 ± 1 | 1.95 ± 0.2 | 388 | |
| t-PA | 545 ± 2 | 0.83 ± 0.04 | 1.3 | 546 ± 1 | 1.58 ± 0.1 | 295 | |