Table 1.
A. Through bond correlation experiments on uniformly labeled 15N sample | |
2D 1H-COSY | 3D HNCO |
2D 1H-15N HSQC | 3D HNHA |
2D 1H-13C HSQC | 3D NH(CA)CO |
2D 1H-TOCSY (75 ms mixing time) | 3D HNCA |
2D 1H-15N TOCSY-HSQC (150 ms mixing time) | 3D HCCH-TOCSY |
3D NH(CO)CA | |
3D CBCANH | |
3D CBCA(C)NH | |
3D C(CO)NH |
B. Through space correlation experiments | |
(NMR) Nuclear magnetic resonance; (TOCSY) total correlation spectroscopy; (NOESY) nuclear Overhauser effect spectroscopy; (HSQC) heteronuclear single quantum correlation; (COSY) correlated spectroscopy. | |
2D 1H-NOESY (200 ms mixing time) | |
3D 15N-edited NOESY (150 ms mixing time) | |
3D 13C-edited NOESY (120 ms mixing time) |