Table 1.
Kinetic and thermodynamic constants determined for CA II/sulfonamide interactions using SPR, ITC, and SFF
| Analysis method | Sulfonamide compound | T (°C) | Expa | ka (M−1s−1) | kd (s−1) | KD (nM) | ΔG° (kcal/mol) | ΔH° (kcal/mol) | ΔS° [cal/(mol K)] |
| SPR | CBS | 25 | 6 | (4.8 ± 0.2) × 104 | 0.0365 ± 0.0006 | 760 ± 30 | −8.3 ± 0.3 | −11.6 ± 0.4 | −11 ± 1 |
| ITC | CBS | 25 | 5 | — | — | 730 ± 20 | −8.4 ± 0.2 | −11.9 ± 0.4 | −12 ± 1 |
| SPR | DNSA | 25 | 8 | (3.9 ± 0.5) × 105 | 0.13 ± 0.01 | 340 ± 40 | −8.8 ± 0.9 | −5.7 ± 0.4 | 11 ± 1 |
| ITC | DNSA | 25 | 2 | — | — | 360 ± 40 | −8.8 ± 0.9 | −4.8 ± 0.4 | 13 ± 1 |
| SFF | DNSA | 25 | 4 | (3.8 ± 0.9) × 105 | 0.16 ± 0.03 | 420 ± 100 | — | — | — |
| SPR | DNSA | 5 | 8 | (1.3 ± 0.2) × 105 | 0.023 ± 0.003 | 180 ± 20 | — | — | — |
| SFF | DNSA | 5 | 4 | (1.4 ± 0.2) × 105 | 0.022 ± 0.005 | 160 ± 40 | — | — | — |
a Number of times the interaction analysis was replicated.
All reported errors were obtained from the standard deviation of the experimental replicates.