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(A) SDS-PAGE of the purified proteins EGIc-HFBI (lane 1), EGIc-HFBII (lane 2). and EGIc (lane 3). Molecular-weight markers are indicated on the side. (B) Size-exclusion chromatography of EGIc-HFBI, EGIc-HFBII, and EGIc using a Superdex 200 column. (Top) The elution volumes of the standards (669, 440, 158, 43, and 13.7 kD). (Bottom) The control EGIc (peak 3) elutes close to the EGIc-HFBII (peak 2) fusion protein, but EGIc-HFBI (peak 1) is a large multimer, putatively a decamer.
