Fig. 3.

(A) Sensograms of EGIc, EGIc-HFBII, and EGIc-HFBI binding to an alkylated gold surface measured by surface plasmon resonance (SPR). The arrow 1 indicates when sample injection started; arrow 2, when sample injection stopped and buffer wash started. The EGIc control does not show any binding to the surface, EGIc-HFBII adsorbs during the injection but desorbs during the wash step, whereas EGIc-HFBI adsorbs during injection and shows a slow desorption during the wash. (B) Sensograms of native HFBI and HFBII binding to an alkylated gold surface measured by SPR. The free hydrophobins do not show the differences in binding seen when the hydrophobins are parts of fusion proteins.