Fig. 2.

SDS-PAGE (10% acrylamide, nonreduced) of α₁-proteinase inhibitor (α₁-PI)-LGR, α₁-PI-PFR, and α₁-PI-LGR-P4′E, and their reactions with C1s, kallikrein, factor XIIa, and activated protein C, as indicated. Each lane contained 5 μg of proteinase and/or inhibitor. The mixtures of proteinase and inhibitor were incubated for 30 min at 37°C prior to stopping the reaction by addition of sample buffer.