Fig. 6.

{¹H}-¹⁵N NOE heteronuclear NOE for the backbone amide nitrogen atoms of the intact (A) and reduced (B) β2-m measured at 500 MHz on the normal scale at pH 2.5 and 37°C. (C) Hydrophobicity of β2-m plotted against residue number. Hydrophobicity was calculated using the scale of Kyte and Doolittle (1982) averaged over a sliding window of seven residues. The locations of secondary structures obtained from the X-ray structure and Cys residues are indicated in (A).