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. Author manuscript; available in PMC: 2008 May 7.
Published in final edited form as: J Urol. 2006 Mar;175(3 Pt 1):1143–1147. doi: 10.1016/S0022-5347(05)00319-8

Figure 2.

Figure 2

Figure 2

Chromatography of PPS isolated from Elmiron capsules (panel a, closed circles) and PPS recovered from urine of an IC patient taking PPS chronically (panel a, open circles) on a column of Sephadex G-50. The column was eluted with 0.1 N pyridine acetate, one mL fractions collected dried and analyzed for PPS by ELISA. The elution profiles from separate experiments are superimposed using peak elution of glucose as reference. Vo indicates the exclusion volume of the column. The average molecular sizes of the Elmiron PPS and PPS excreted by the IC patient were estimated based on calibration of the column with a heparin fragment (3500 Daltons) and sialyl saccharides of 966, 675 and 309 Daltons as calibration standards (panel b).