Table 1.
Peptide fragments identified by MALDI-MS for ScrY-porin and ScrY(Δ1-62) by trypsin digestion
| Molecular Mass | ||||
| Peptide fragment | Sequence/PSDa | Calculated | Measured (ScrY) | Measured (ScrY(Δ1-62)) |
| T1 | QTDISTIEAR (1-10) | 1133.58 | 1133.38 | — |
| T2–6 | LNALEKRLQEAENRAQTAENR (11–37) | 2982.3 | 2980.8 | — |
| T4 | LQEAENR (18–24) | 859.4 | 859.3 | — |
| T5 | AQTAENR (25–31) | 789.3 | 789.2 | — |
| T5–6 | AQTAENRAGAAEK (25–37) | 1316.7 | 1317.0 | — |
| T6–7 | AGAAEKK (32–38) | 673.8 | 674 | — |
| T7–9 | KVQQLTAQQQKNQNSTQEVAQR (38–59) | 2555.8 | 2555.0 | — |
| T9 | NQNSTQEVAQRa (49–59) | 1274.6 | 1274.4 | — |
| T9–10 | NQNSTQEVAQRTAR (49–62) | 1602.8 | 1603.6 | — |
| T10–11 | TARLEK (60–65) | 717.4 | 717.8 | — |
| T12–13 | KADDK (66–70) | 576.3 | 576.0 | — |
| T14 | SGFEFHGYARa (71–80) | 1170.5 | 1170.9 | — |
| T14–15 | SGFEFHGYARSGVIMNDSGASTK (71–93) | 2418.1 | 2418.0 | — |
| T16 | SGAYITAGETGGAIGa (94–110) | 1577.7 | 1577.4 | 1577.8 |
| T17 | LGNQADTYVEMNLEHKa (111–126) | 1861.8 | 1861.6 | — |
| T19–20 | FKVMVADGQTSYNDWTASTSDLNVR (137–161) | 2805.3 | 2806.1 | — |
| T20 | VMVADGQTSYNDWTASTSDLNVR (139–161) | 2530.1 | 2529.8 | 2530.1 |
| T21 | QAFVELGNLPTFAGPFK (162–178) | 1835.9 | 1834.6 | 1836.0 |
| T26 | WNDGLRa (217–222) | 760.3 | 760.2 | 760.4 |
| T27 | SNFSLYGRa (223–230) | 943.4 | 943.2 | 943.1 |
| T28–29 | NFGDIDDSSNSVQNYILTMNHFAGPLQMMVSGLRAK (231–266) | 3972.5 | 3974.0 | — |
| T30–31 | DNDERK (267–272) | 776.3 | 775.9 | — |
| T34–35 | DGSSKTALLYGHGLGAEVK (304–322) | 1902.0 | 1903.1 | — |
| T36 | GIGSDGALRPGADTWR (323–338) | 1628.8 | 1628.7 | 1629.1 |
| T38–39 | SKDR (362–365) | 505.3 | 505.3 | 505.2 |
| T40 | YADGDSYQWATFNLR (366–380) | 1806.8 | 1806.4 | — |
| T40–41 | YADGDSYQWATFNLRIQAINQNFALAYEGSYQYMDLKPEGYNDR (366–410) | 5312.5 | 5313.0 | — |
| T42–43 | QAVNGSKFYKLTFAPTFK (411–427) | 1919.0 | 1918.2 | 1918.0 |
| T43–44 | LTFAPTFKVGSIGDFFSRPEIR (420–441) | 2485.3 | 2485.0 | 2483.9 |
| T44 | VGSIGDFFSRPEIR (428–441) | 1579.8 | 1579.7 | 1578.1 |
| T45–46 | FYTSWMDWSKK (442–452) | 1478.6 | 1478.3 | 1479.1 |
a PSD-sequencing was performed on the underlined fragments.