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. 2002 Jun;11(6):1435–1441. doi: 10.1110/ps.0202602

Table 1.

Main biochemical parameters for the AIIA/NO3−, complex and for the mutants K300Q and K300R

Enzyme Anion bound Kd kcal
mM s−1
AHA Cl− 5.9 609
AHA NO3− 1.9 274
K300Q none — 77
K300R Cl− 44 338

Activation kinetics and activities using 4-nitrophenyl-α-d-maltoheptaoside-4, 6-O-ethylidene as substrate (Feller et al. 1996).