Table 5.
Global energy minimum sequence found by PDA using optimized parameters for a core design of hGHa (Design) and experimentally tested sequences (CORE1, CORE2, CORE3)
| Positionb | Wild type | Design | CORE1 | CORE2 | CORE3 |
| 6 | Leu | —c | — | — | — |
| 10 | Phe | — | — | — | — |
| 13 | Ala | Val | Val | Val | Val |
| 17 | Ala | — | — | — | — |
| 20 | Leu | — | — | — | — |
| 24 | Ala | — | — | — | — |
| 27 | Thr | Val | Val | Val | Val |
| 28 | Tyr | Phe | — | — | Phe |
| 31 | Phe | — | — | — | — |
| 36 | Ile | — | — | — | — |
| 44 | Phe | — | — | — | — |
| 54 | Phe | Tyr | — | — | Tyr |
| 55 | Ser | Ala | — | Ala | Ala |
| 58 | Ile | — | — | — | — |
| 73 | Leu | — | — | — | — |
| 75 | Leu | — | — | — | — |
| 76 | Leu | — | — | — | — |
| 78 | Ile | — | — | — | — |
| 79 | Ser | Ala | Ala | Ala | Ala |
| 80 | Leu | — | — | — | — |
| 81 | Leu | — | — | — | — |
| 82 | Leu | — | — | — | — |
| 83 | Ile | — | — | — | — |
| 85 | Ser | Ala | — | Ala | Ala |
| 90 | Val | Ile | Ile | Ile | Ile |
| 93 | Leu | — | — | — | — |
| 96 | Val | — | — | — | — |
| 97 | Phe | — | — | — | — |
| 105 | Ala | — | — | — | — |
| 110 | Val | — | — | — | — |
| 114 | Leu | Met | — | — | — |
| 117 | Leu | — | — | — | — |
| 121 | Ile | — | — | — | — |
| 124 | Leu | — | — | — | — |
| 157 | Leu | — | — | — | — |
| 161 | Gly | Met | Met | Met | Met |
| 162 | Leu | — | — | — | — |
| 163 | Leu | — | — | — | — |
| 166 | Phe | — | — | — | — |
| 170 | Met | — | — | — | — |
| 173 | Val | — | — | — | — |
| 176 | Phe | — | — | — | — |
| 177 | Leu | — | — | — | — |
| 180 | Val | — | — | — | — |
| 184 | Ser | Ala | Ala | Ala | Ala |
a Protein Data Bank structure 3HHR.
b The residues are numbered as in 3HHR file from Brookhaven Protein Databank.
c "—" indicates the wild-type residue.