Fig. 4.

Chemical Shift Index, Δδ_Hα = δ_Hα(observed) − δ_Hα(unfolded reference peptide 4), for the inner hydrogen-bonded strand residues of peptide 1 in 9:1 H₂O:D₂O and 100 mM sodium deuteroacetate buffer at pH 3.8 (uncorrected) and 3°C with various solvent additives (solid bars: 6 M urea; open bars: no additive; horizontal stripes: 30% (v/v) TFE; vertical stripes: 50% (v/v) methanol), and for the same residues of cyclic peptide 5 (dotted bars, no additive). In each case, the reference data for 4 were obtained in the appropriate solvent system.