Fig. 2.

Recombinant DNA plasmids purified from JM109(DE3). Eight different fusion protein expression vectors are indicated above. Two independent clones from each construct were isolated for characterization (lanes A and B). Plasmid DNAs were purified in a 96-well format using Millpore's Motage plasmid mini-prep kit; 3–5 μL mini-prep DNA was restriction digested with EcoRI and XhoI and separated in 0.8% agarose gel. A 1-kb DNA ladder (from MBI Fermentas, USA) was used as marker (M) and shown in the far left lane. The expected sizes (in base pair) of the desirable restriction fragments of two different target genes are indicated on the right of the figure.