Fig. 2.

³⁵S-Labeled HlyII and HlyII(ΔCT) synthesized by coupled in vitro transcription and translation (IVTT) with an S30 extract from E. coli. An autoradiogram of a 10% SDS-polyacrylamide gel is shown. Lane 1, HlyII translated in the absence of rRBCM; lane 2, HlyII(ΔCT) translated in the absence of rRBCM; lane 3, HlyII translated in the presence of rRBCM; lane 4, HlyII(ΔCT) translated in the presence of rRBCM; lane 5, HlyII incubated in the presence of freshly prepared liposomes for 1 h at room temperature; lane 6, HlyII(ΔCT) incubated in the presence of liposomes. In the samples used in lanes 3 and 4, the rRBCM were washed before electrophoresis of the bound protein. In the samples used in lanes 5 and 6, the entire sample was loaded onto the gel. The high molecular weight bands (arrows) represent oligomerized HlyII and HlyII(ΔCT).