Fig. 3.

Conformational states of HlyII, HlyII(ΔCT) and αHL examined by limited proteolysis. HlyII, HlyII(ΔCT) and αHL were translated in the presence of rRBCM. The membranes were washed, treated with proteinase K, solubilized and subjected to electrophoresis in a 10% SDS-polyacrylamide gel prior to autoradiography. Lanes 1–4, HlyII; lanes 5–8, HlyII(ΔCT); lanes 9–12, αHL. The final proteinase K concentrations were: lanes 1, 5, and 9, 0 μg mL⁻¹; lanes 2, 6, and 10, 5 μg mL⁻¹; lanes 3, 7, and 11, 50 μg mL⁻¹; lanes 4, 8, and 12, 500 μg mL⁻¹.