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. 2002 Jul;11(7):1813–1824. doi: 10.1110/ps.0204002

Fig. 7.

Fig. 7.

Subunit stoichiometry of the HlyII pore. (A) Constructs used in the study. The maroon block represents the 94-residue C-terminal extension. The oligoaspartate (D8) tail is shown in red. (B) SDS-polyacrylamide gel electrophoresis of heteromers generated from WT HlyII and/or HlyII-D8. (C) Heteromers generated from αHL and/or αHL-D8. (D) Heteromers generated from HlyII(ΔCT) and/or HlyII(ΔCT)-D8. The DNA constructs were mixed at various molar ratios, as indicated in (B–D), and cotranslated in the presence of rRBCM. The membranes were washed, solubilized in sample buffer without heating, and subjected to electrophoresis in a 5% gel. Autoradiograms of dried gels are shown. The results in B are from a long gel (36 cm), which was run for 72 h at 50 V.