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. 2002 Jul;11(7):1813–1824. doi: 10.1110/ps.0204002

Table 1.

Single-channel properties of wild-type and engineered HlyII pores

Protein g (pS)a n I+80/I−80b Vr(mV); PK+/PCl−c
HlyII(WT) 637 ± 51 180 0.566 ± 0.004 −18.0 ± 1.3; 0.29 (n = 4)
HlyII(ΔCT) 636 ± 73 142 0.583 ± 0.001 n.d.
HlyII(ΔCT)-D8 610 ± 54 273 0.591 ± 0.032 −19.3 ± 1.2; 0.26 (n = 3)
[HlyII(ΔCT)]6/ [HlyII(ΔCT)-D8]1 642 ± 62 144 0.691 ± 0.077 n.d.

a Recordings were made at −40 mV in 1 M KCl, 5 mM HEPES, pH 7.4. Single-channel conductances were determined by fitting the peaks in amplitude histograms to Gaussian functions (e.g., Figs. 5, 8 ▶ ▶).

b Rectification ratios were determined from three or more experiments.

c The reversal potentials (Vr) and permeability ratios (PK+/PCl−) are mean values ± SD determined with the following buffers: cis, 1 M KCl, 5 mM HEPES, pH 7.4; trans, 0.2 M KCl, 5 mM HEPES, pH 7.4. n.d., not determined.