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Analytical size-exclusion chromatography of Ejl amidase at increasing choline concentrations. Variation of the self-association state of the Ejl amidase on choline concentration (20 mM phosphate buffer; pH 8.0). The arrows indicate the exclusion volume and the elution positions of the different Ejl species expressed as Kav values: 0.35 (monomer), 0.24 (dimer), and 0.13 (tetramer). The initial Ejl concentration was 5.5 μM.
