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Far-UV CD spectra and Amide I band (D2O) of Ejl in the absence and presence of choline. (A) CD spectra of Ejl in the absence (open circles) and presence of 20 mM choline (filled circles) (buffer: 20 mM phosphate pH 8.0; T = 25°C). (B) Comparison of the experimental amide I band of Ejl in the absence (thin trace) and presence of choline (thick trace), in arbitrary units. (C) Amide I band decompositions of the Ejl amidase; the parameters derived for the components are shown in Table 1.
