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. 2002 Aug;11(8):1926–1936. doi: 10.1110/ps.5000102

Fig. 2.

Fig. 2.

Determination of the oligomeric state: gel filtration chromatography results. (A) Elution profile of alcohol dehydrogenase (1), BSA (2), carbonic anhydrase (3), and cytochrome b (4) in 50 mM TrisHCl, 150 mM NaCl, pH 8.0. Inset shows the calibration curve. KAV is calculated using equation 1. (B) Elution profile of alcohol dehydrogenase (1), BSA (2), carbonic anhydrase (3), and cytochrome b (4) in 50 mM TrisHCl, 150 NaCl, pH 8.0 in 0.1% Zwittergent 3–12. Insert shows the calibration curve calculated as shown above. (C) Elution profile of GR-LBD in 50 mM TrisHCl, 150 mM NaCl in the absence (solid line), or presence of 0.1% Zwittergent 3–12 (dashed line).