Fig. 3.

Crosslinking of native and heat-denatured BfAChE by hypericin as demonstrated by SDS-PAGE. Electrophoresis was performed as described previously, but under nonreducing conditions, using a 3.5–15% acrylamide gradient. Lane 1, native TcAChE dimer (ca. 130 kD). Lane 2, native BfAChE monomer. Lane 3, native BfAChE + hypericin, irradiation for 10 min. Lanes 4–6, heat-denatured BfAChE + hypericin, irradiation for 10 min; heat denaturation was performed for 2, 5, and 10 min, respectively, at 50°C, in buffer 1. Lane M, high molecular weight markers.