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Thioflavin assay. The persistence of fibrillar structure in the proteolysis experimental conditions (pH 7.5 ▴), in β2-m fibrillogenesis conditions (pH 4 O), and in the buffer used to solubilize the proteolysed fibrils (40% CH3CN 0.4% TFA ▪ ) was monitored by thioflavine assay.