Fig. 5.

Stability of the kinetic intermediate compared to the native state. The stability of the kinetic intermediate (open circles) was determined from the GdnHCl dependence of the amplitude of the fluorescence detected fast refolding reaction. The fraction unfolded for the fast folding reaction was determined by the change in the amplitude from the pretransition baseline amplitude compared to the total change of amplitude for the fast folding reaction. The pretransition baseline amplitude was obtained from the best straight-line fit of the fast folding amplitudes below 2 M GdnHCl. The stability of the native state (filled circles) was obtained from the equilibrium denaturation results obtained by fluorescence detection as illustrated in Figure 2 ▶.