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Binding specificity and affinity in mutants of glutamate/aspartate BP
| Kd (μM) | |||||
| Mutant | Fluorophore | Glu | Asp | Gln | Asn |
| Q123C | Fluorescein | 0.75 | 1.8 | 49 | 96 |
| F126C | Acrylodan | 82 | 115 | ||
| F126C | Fluorescein | 1707 | 2000 | ||
| F126C | JPW4045 | 903 | 1497 | ||
| T129C | NBD | 0.019 | 0.061 | 12.1 | 5.4 |
| T129C | JPW4039 | 0.093 | 0.035 | 23 | |
| F131C | JPW4039 | 0.15 | |||
| A207C | NBD | 119 | 454 | ||
| A210C | JPW4042 | 0.10 | |||
