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Binding of ATP to YajQ as determined by high-sensitivity isothermal titration calorimetry. (A) The binding isotherm at 25°C. The shape of the binding isotherm corresponds to that obtained with C = 2 (Wiseman et al. 1989). (B) The enthalpy (ΔH, circles), entropy (TΔS, squares), and free energy (ΔG, triangles) changes for binding of ATP to YajQ versus temperature.
