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Elution profiles of the wild-type Tva LDL-A module and its W33 mutants by reverse-phase high-performance liquid chromatography (HPLC). The purified proteins were folded in vitro either in the presence (A, C, E, G, I, and K ) or absence (B, D, F, H, J, and L) of calcium, and then eluted by reverse-phase HPLC. X-axis, elution time (min), Y-axis, absorbance (280 nm).
