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. 2002 Nov;11(11):2596–2605. doi: 10.1110/ps.0219802

Fig. 4.

Fig. 4.

Fig. 4.

Fig. 4.

Fig. 4.

2D [1H-15N]-HSQC spectra of W33E and W33G mutants. The purified 15N-labeled proteins were folded in vitro in the presence of calcium and were further purified by reverse-phase high-performance liquid chromatography. Three fractions were collected for W33E mutant protein (A), and W33G mutant protein (C), respectively. The two-dimensional [1H-15N]-HSQC spectra of W33E mutant protein (B), and that of W33G mutant protein (D) were acquired either in the presence (a, c, and e) or absence (b, d, and f) of calcium.