Fig. 5.

Residues of the Tva LDL-A module affected by amino-acid substitutions of W33. The residues within the Tva LDL-A module whose chemical shifts have been changed as a result of the W33 substitutions in the [¹H-¹⁵N]-HSQC spectra are labeled in blue. W33F: Y29, L34 and D46; W33K: Y29 and D46; W33E (peak fraction 1): Y29 and D46; and W33A: R17, A24, Y29, D32, L34, and D46. W33G mutation caused changes in chemical shifts of numerous residues (not shown). The likely ligand contact residues, H38 and W48, are shown in green. The Tva LDL-A module structure is from Wang et al. (2002).