Fig. 2.

Monte Carlo simulation (•) and experimental data (□) for unfolding forces of (C47S, C63S I27)₅ as a function of the unfolding event number # (see Fig. 1 ▶). Data are expressed as a fraction of the sum of the unfolding forces in order to combine many experimental data sets. Experimental data are expressed as a weighted mean ± weighted standard error of the mean. (Inset) Relative force–frequency histograms for #1 and #3 for all data sets (n = 104). Solid lines are fits to a single (#3) or a double (#1) Gaussian function. The points (×) and (▵) are the modes obtained from the fit to the distribution for #1.