Figure 3.
(a) Graphical presentation of PfTRAP mutants. (b) Binding of purified recombinant TRAP ECD and its mutants to heparin (100 μg) coated on ELISA plates. They were incubated with equimolar amounts of mutants. Binding was evaluated in an ELISA based assay using PfTRAP antibodies. Results are the mean SD of n = 3 experiments. (c) Binding of the purified recombinant TRAP ECD and its mutants to HepG2 cells. HepG2 cells (approx. 105) cells were incubated with ECD and its mutants in equimolar amounts. Binding was evaluated in an ELISA based assay using PfTRAP antibodies. Results are the mean ± SD of n = 6 experiments.