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. 2008 Apr 22;7:63. doi: 10.1186/1475-2875-7-63

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Copyright © 2008 Akhouri et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Recombinant PfTRAP ECD is phosphorylated at multiple residues. PfTRAP ECD was either incubated with HepG2 cell or HEK 293 cell lysate and immunoprecipitated using PfTRAP antibodies. Phosphorylation status was analyzed by western blotting using monoclonal antibodies against phosphorylated serine threonine and tyrosine (a, b and c). Lanes 4 and 5 show the phosphorylated PfTRAP ECD in presence of HEK 293 and HepG2 cell lysate respectively. HepG2 lysate alone (lane 1, panels a, b and c), HEK293 lysate alone (lanes 2 of a, b and c) were taken as controls. Autophosphorylation of ECD was also analyzed (lane 3, panels a, b and c) and none was observed.