Table 2.
Forward Primer | Reverse Primer | Residue mutated |
P12 | P13 | C42G |
P14 | P15 | C55G |
P16 | P17 | T131A |
P18 | P19 | D162A |
P20 | P21 | C205G |
P22 | P23 | C212G |
P24 | P25 | S251A |
P26 | P27 | S285G |
P28 | P29 | R307G |
P30 | P31 | S416A |
P32 | P33 | T470A |
Forward Primer | Reverse Primer | Residue mutated |
P12 | P13 | C42G |
P14 | P15 | C55G |
P16 | P17 | T131A |
P18 | P19 | D162A |
P20 | P21 | C205G |
P22 | P23 | C212G |
P24 | P25 | S251A |
P26 | P27 | S285G |
P28 | P29 | R307G |
P30 | P31 | S416A |
P32 | P33 | T470A |