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. 2008 May 12;205(5):1077–1086. doi: 10.1084/jem.20072416

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© 2008 Machado et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Restoration of TRAF expression overcomes LX-mediated modulation of myeloid cell cytokine production. (A) RAW 264.7 cells were transfected with TRAF2, TRAF3, TRAF6, or control plasmids, followed by quantification of TRAF2, TRAF3, TRAF6, and actin by Western blot. RAW 264.7 cells, transfected and treated with LXA₄ as noted in B, were stimulated with TNF (100 ng/ml), CD40L, STAg or LPS (all at 1 μg/ml). 24 h later, IL-12p70 was quantified by FACS. Data shown represent the means ± the SD of triplicate samples and are representative of at least two independent experiments with similar results. *, P < 0.01, PBS versus LX treatment.

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