Table III.
PCR primers in 5′-3′ direction
| Gene | Sequencea | PrimerBank ID | Product size (bp) |
|---|---|---|---|
| ALP | F-CCAACTCTTTTGTGCCAGAGA R-GGCTACATTGGTGTTGAGCTTTT |
6671533a1 | 110 |
| Col1 α1 | F-GCTCCTCTTAGGGGCCACT R-CCACGTCTCACCATTGGGG |
34328108a1 | 103 |
| L32b | F-CACAATGTCAAGGAGCTGGAAGT R-TCTACAATGGCTTTTCGGTTCT |
NA | 100 |
| OCN | F-CTGACCTCACAGATGCCAAGC R-TGGTCTGATAGCTCGTCACAAG |
13811695a1 | 187 |
| OPN | F-AGCAAGAAACTCTTCCAAGCAA R-GTGAGATTCGTCAGATTCATCCG |
6678113a1 | 134 |
| OSX | F-ATGGCGTCCTCTCTGCTTG R-TGAAAGGTCAGCGTATGGCTT |
18485518a1 | 156 |
| OPG | F-GGGCGTTACCTGGAGATCG R-GAGAAGAACCCATCTGGACATTT |
31543882a2 | 125 |
| RANKL | F-CCAGCTATGATGGAAGGCTCA R-CGTACAGGTAATAGAAGCCA |
NA | 223 |
NA, not available.
a
F, forward; R, reverse.
b
Designed with Primer Express software, version 2.0 (PerkinElmer).