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. 2008 May 12;205(5):1155–1171. doi: 10.1084/jem.20072509

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© 2008 Rolny et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — SEMA3B knock down in tumor cells affects IL-8 production and monocyte recruitment. (A) RNA was collected from LoVo, A549, HeLa, and Sk-Mel-2 cells transduced with shRNA against SEMA3B (to suppress gene expression; shSEMA3B) and unrelated sequences (shCTR). Real-time PCR was performed using Taqman probes for SEMA3B (results shown at the bottom) and IL-8 (in the graph). Expression levels were normalized to controls for each cell line and are represented as fold changes. Data shown are the mean ± SEM. (B) Monocyte migration to CM derived from Sk-Mel-2 melanoma cells transduced with shRNAs. IL-8 neutralizing antibodies (MAB208) or unrelated control antibodies (anti-CD19) were included in the lower chamber. Knock down of SEMA3B expression in Sk-Mel-2 cells abolished IL-8–dependent monocyte-attracting activity in the CM. The number of migrated monocytes represents the mean ± SEM of two independent experiments. **, P < 0.01.