Table I.
T cell lineage precursor frequency in early stages of T cell development isolated from CD45.1+ newborn donor thymic lobes either 4 or 6 d after transplantation under the kidney capsule of CCR9EGFP/+//CD45.1/CD45.2 F1 recipient mice
| T cell progenitor frequency−1 (95% confidence interval)
|
|||
|---|---|---|---|
| Time of sorting after transplantation |
Sorted cell type | CD45.2+ CCR9EGFP+ (recipient-derived) |
CD45.2− CCR9EGFP− (donor-derived) |
| Day 4 | EGFP-pos ETPa | 24.5 (5.8–103.7) | 5.4 (2.6–11.6) |
| ETP | 43.5 (10.6–176.8) | 2.5 (1.6–4) | |
| DN1c | ∞ | 17.5 (2.4–129.3) | |
| DN1d | ∞ | ∞ | |
| DN1e | ∞ | 1,323 (320–5,481) | |
| CLP-2 | ∞ | ∞ | |
| CTP | ∞ | 168 (40.5–698) | |
| Day 6 | EGFP-pos ETP | 5.9 (3.8–9.2) | ∞ |
| ETP | 22.2 (14.2–34.5) | 28 (17.2–45.6) | |
| DN1c | ∞ | 37.7 (12.1–117.5) | |
| DN1d | ∞ | 439 (107–1,800) | |
| DN1e | ∞ | ∞ | |
| CLP-2 | ∞ | 58.8 (25.4–135.9) | |
| CTP | ∞ | ∞ | |
a
4 d after transplantation, the vast majority of ETPs are donor derived, and a defined EGFP+-recipient ETP population that can be gated for is not discernible.