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Exogenous Tiam1 promotes migration and invasion of pancreatic cancer cells. (A) Panc-1 2G6 cells (low integrin β4 expression) were transiently transfected with a full-length construct of Tiam1 and then assessed for chemotactic migration on laminin-1 and collagen invasion toward 50 ng/ml of HGF. *P < .001 compared to pcDNA transfected cells. (B) Immunoblot of Tiam1 to confirm expression of full-length Tiam1 and actin as loading control. Transfection efficiency was approximately 60%.
