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. 2008 May;10(5):489–500. doi: 10.1593/neo.08200

Figure 1.

Figure 1

The expression of TGF-α and EGFR by SW620CE2, SW620CE2 nontargeting shRNA, and SW620CE2 TGF-α shRNA cells growing in culture. RT-PCR (A), ELISA (B), and Western blot analysis (D) reveal that using the lentiviral system, the expression of TGF-α was decreased in the SW620CE2 TGF-α shRNA cells compared to the other cells, SW620CE2 WT and SW620CE2 nontargeting shRNA cells that expressed minimal levels of EGFR mRNA (C) and protein (D). HT29 human colon carcinoma cells [27] were used as the positive control for EGFR expression.