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. 2008 May 21;3(5):e2231. doi: 10.1371/journal.pone.0002231

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A. Average data of calpain-1 transcriptome. B. Western blots for calpain-1 protein of rat intact aortae (upper panel); average data (lower panel). C. Dual en face fluorescence staining for α-SMA (green), calpain-1 (red) in the medial aortic sections from young (upper panels) and old rats (lower panels). Nuclei were counterstained with DAPI (blue). Merged image is depicted in right panel (yellow-blue). Magnification: ×400. D. Western blots for calpain substrate α-II spectrin from aortic lysates (left panels). Average cleaved spectrin fraction (right panel). *p<0.05, old vs. young.