Table 2. Primary antibodies used for immunostaining (IS) and Western blotting (WB).

Antibody	Species	Titer for IS	Titer for WB	Source
Calpain-1	Rabbit	1∶100	1∶2500	Sigma, St. Louis, MO
α-II spectrin	Mouse		1∶3000	BioMol, Plymouth Meeting, PA
α-SMA	Mouse	1∶80		DAKO, Glostrup. Denmark
Ang II	Rabbit	1∶50		Peninsula Laboratories, Inc., Dan Carlos, CA