A NB map of rows 5, 6 and 7, from S3 to S5 waves of delamination from early stage 10 (e10) to late stage 11 (l11), with indicated expression of EN (in black), GsbN (in pink), Eagle (in green), Collier (in yellow), and Klumpfuss (in blue). B Rescue experiments involving the reintroduction of GsbN into cells that are affected in the transheterozygous (Df enX31/Df gsbX62) embryos shown in (2), to compare to wild-type VNC shown in (1). Flat preparations are shown of stage 15 embryos labeled with a Cy3-conjugated anti-HRP antibody to visualize the VNC architecture (red). According to the diagram shown in A, GsbN expression was driven: (3) with eagle-Gal4 in NB 6-4 and NB 7-3; (4) with collier-Gal4 in several NBs, including NB 6-4, but not NB 7-3; and (5) with Klumpfuss-Gal4 in several NBs, including NB 7-3, but not NB 6-4.